A physiological characterization in controlled bioreactors reveals a novel survival strategy for <scp> <i>Debaryomyces hansenii</i> </scp> at high salinity

Debaryomyces hansenii is traditionally described as a halotolerant non-conventional yeast and has served model organism for the study of osmotolerance salt tolerance mechanisms in eukaryotic systems past 30 years. However, unraveling D. hansenii's biotechnological potential always been difficult due to persistent limitations availability efficient molecular tools this yeast. Additionally, there lack consensus contradictory information along recent years that limits comprehensive understanding its central carbon metabolism, mainly physiological studies controlled monitored environments. Moreover, little consistency culture conditions (media composition, temperature, pH among others) used by different groups, which makes it complicated when trying get prevalent conclusions on behavioral patterns. In work, we present first time characterization batch cultivations using highly lab-scale bioreactors. Our findings contribute more complete picture metabolism external influence yeast's ability tolerate high Na+ K+ concentrations, pointing differential effect both salts, well positive cell performance low environmental values are combined with sodium concentration media. Finally, novel survival strategy at very salinity (2 M) proposed yeast, outcomes use industrial biotechnology applications. Take Away High concentrations stimulate respiration hansenii. Sodium exerts stronger impact than potassium. ?max higher combination pH, salt, temperature. Concentrations 2 M result slower growth but increased biomass yield. The salts enhanced glucose concentration.